Journal: Journal of Vascular Surgery

Article Title: S-phase kinase-associated protein-2 (Skp2) promotes vascular smooth muscle cell proliferation and neointima formation in vivo

doi: 10.1016/j.jvs.2009.07.066

Figure Lengend Snippet: Neointimal thickening 28 days after common carotid ligation in Skp2 +/+ and Skp2 −/− mice. Carotid ligation was performed on wild-type or homozygous Skp2-null mice. A , Representative longitudinal sections of from both groups 28 days post-ligation. Arrows locate ligation position. B , Quantification of neointimal areas. The comparison was made using Mann-Whitney test.

Article Snippet: Antibodies for Skp2 were obtained from US Biological Inc (Swampscott, Mass) and Invitrogen (Carlsbad, Calif).

Techniques: Ligation, Comparison, MANN-WHITNEY

Journal: Journal of Vascular Surgery

Article Title: S-phase kinase-associated protein-2 (Skp2) promotes vascular smooth muscle cell proliferation and neointima formation in vivo

doi: 10.1016/j.jvs.2009.07.066

Figure Lengend Snippet: DN-Skp2 up regulates p27 Kip1 levels, inhibits VSMC proliferation and neointima formation in balloon-injured rat common carotid arteries. Balloon-injured rat common carotid arteries were infected with either Ad:βGal (control) or Ad:DN-Skp2 by the luminal retention of 100 μl adenoviral solution (1 × 10 9 pfu/mL) for 30 minutes. The animals were injected with BrdU (25 mg/kg) at 17, 9, and 1 hour before euthanasia on the day 4 post balloon-injury. A , Representative pictures of arterial sections undergoing staining for p27 Kip1 , BrdU, phospho-Rb, p21 Cip1 , 4 days post injury and hematoxylin and eosin staining 14 days post injury, as indicated. Scale bars = 50 μm. Arrows indicate labeled cells. B , Quantified results of the immunohistochemistry and I/M ratios. Data are expressed as % of labeled cells. ** indicates P < .01, * indicates P < .02.

Article Snippet: Antibodies for Skp2 were obtained from US Biological Inc (Swampscott, Mass) and Invitrogen (Carlsbad, Calif).

Techniques: Infection, Control, Injection, Staining, Labeling, Immunohistochemistry

Journal: Journal of Vascular Surgery

Article Title: S-phase kinase-associated protein-2 (Skp2) promotes vascular smooth muscle cell proliferation and neointima formation in vivo

doi: 10.1016/j.jvs.2009.07.066

Figure Lengend Snippet: Skp2 over expression down regulates p27 Kip1 levels and increases VSMC proliferation in minimally-injured rat common carotid arteries. After filament de-endothelialization, carotid arteries were infected with Ad:βGal or Ad:Skp2. A , Representative pictures of both groups. Carotid arterial sections were subjected to immunohistochemistry for the staining of Skp2, p27 Kip1 , phospho-Rb, p21 Cip1 and BrdU 4 days post infection. Of note, Skp2- and BrdU-labeled cells were detected in Ad:βGal infected vessels mostly over the superficial layer of media ( arrows ), whereas those infected with Ad:Skp2 tended to have Skp2 and BrdU-positive cells trans-medially. Scale bars = 50 μm. Arrows indicate labeled cells. B , Quantification of the immunohistochemistry. Data are expressed as the percentage of labeled cells. ** indicates P < .01, * indicates P < .02.

Article Snippet: Antibodies for Skp2 were obtained from US Biological Inc (Swampscott, Mass) and Invitrogen (Carlsbad, Calif).

Techniques: Over Expression, Infection, Immunohistochemistry, Staining, Labeling

Journal: Journal of Vascular Surgery

Article Title: S-phase kinase-associated protein-2 (Skp2) promotes vascular smooth muscle cell proliferation and neointima formation in vivo

doi: 10.1016/j.jvs.2009.07.066

Figure Lengend Snippet: Wild-type Skp2 enhances neointimal thickening in minimally-injured rat carotid arteries. Carotid arteries were treated as in . Eight days after filament de-endothelialization, the carotids from both treatment groups were subjected to hematoxylin and eosin staining as described in . A , Representative pictures of both groups. Scale bar = 100 μm. B , Quantified results of I/M ratios.

Article Snippet: Antibodies for Skp2 were obtained from US Biological Inc (Swampscott, Mass) and Invitrogen (Carlsbad, Calif).

Techniques: Staining